Blocking pathogen transmission at the source: reservoir targeted OspA-based vaccines against Borrelia burgdorferi

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Blocking pathogen transmission at the source: reservoir targeted OspA-based vaccines against Borrelia burgdorferi

Control strategies are especially challenging for microbial diseases caused by pathogens that persist in wildlife reservoirs and use arthropod vectors to cycle amongst those species. One of the most relevant illnesses that pose a direct human health risk is Lyme disease; in the US, the Centers for Disease Control and Prevention recently revised the probable number of cases by 10-fold, to 300,00...

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Reservoir targeted vaccine against Borrelia burgdorferi: a new strategy to prevent Lyme disease transmission.

A high prevalence of infection with Borrelia burgdorferi in ixodid ticks is correlated with a high incidence of Lyme disease. The transmission of B. burgdorferi to humans can be disrupted by targeting 2 key elements in its enzootic cycle: the reservoir host and the tick vector. In a prospective 5-year field trial, we show that oral vaccination of wild white-footed mice resulted in outer surface...

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Borrelia burgdorferi OspA is an arthropod-specific transmission- blocking Lyme disease vaccine

Borrelia burgdorferi, the spirochetal agent of Lyme disease, is transmitted by Ixodes ticks. A vaccine based on B. burgdorferi outer surface protein (Osp) A protects mice from spirochete infection. Here we report on the expression of OspA on spirochetes inside engorging ticks and relate OspA expression to antispirochetal immunity. Spirochetes in the gut of unfed nymphal ticks were stained by an...

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Protection against antigenically variable Borrelia burgdorferi conferred by recombinant vaccines

Due to local variation in the antigenicity of the agent of Lyme disease (Borrelia burgdorferi), a vaccine derived from any one isolate of this spirochete may fail to protect against the heterogeneous population of organisms that may be present in an enzootic focus. Accordingly, we determined whether antigenically variable spirochetes delivered by naturally infected ticks, collected from a site ...

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ospa sequence comparison and protection against borrelia burgdorferi infection in gerbils by recombinant ospa protein

lyme borreliosis is a tick-born disease caused by the spirochete borrelia burgdorferi. the aim of this research was molecular typing of different strains of b. burgdorferi and protective efficacy of outer surface protein in animal model. seven strains of b. burgdorferi isolated from skin and csf of lyme disease patients were compared using pcr- rflp as well as dna and amino acid sequences. the ...

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ژورنال

عنوان ژورنال: Frontiers in Cellular and Infection Microbiology

سال: 2014

ISSN: 2235-2988

DOI: 10.3389/fcimb.2014.00136